2024

Paper

• "Metabolic RNA Labeling and Translating Ribosome Affinity Purification for Measurement of Nascent RNA Translation"
  Hirotatsu Imai, Akio Yamashita
  Bio-protocol 14, 20, e5091 (2024).

  • DOI: 10.21769/BioProtoc.5091

2023

Paper

• "Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification"
  Imai H, Utsumi D, Hidetsugu T, Takahashi K, Kuroyanagi H, Yamashita A
  Nucleic Acids Research 51, 11, e76 (2023).

  • URL
  • DOI: 10.1093/nar/gkad545

2022

Paper

• "The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation"
  Yang L, Lee KM, Yu CWH, Imai H, Choi AKH, Banfield DK, Ito K, Uchiumi T, Wong KB
  Nucleic Acids Research 50, 5335–5348 (2022).

  • DOI: 10.1093/nar/gkac292

2020

Paper

• "Arabidopsis ROOT PHOTOTROPISM2 Is a Light-Dependent Dynamic Modulator of Phototropin1"
  Kimura T, Tsuchida-Mayama T, Imai H, Okajima K, Ito K, Sakai T
  The Plant Cell 32, 2004-2019 (2020).

  • DOI: 10.1105/tpc.19.00926
• "Direct visualization of translational GTPase factor pool formed around the archaeal ribosomal P-stalk by high-speed AFM"
  Imai H, Uchiumi T, Kodera N
  Proceedings of the National Academy of Sciences 117, 32386-32394 (2020).

  • DOI: 10.1073/pnas.2018975117

2019

Paper

• "Switch of the interactions between the ribosomal stalk and EF1A in the GTP-and GDP-bound conformations"
  Maruyama K, Imai H, Kawamura M, Ishino S, Ishino Y, Ito K, Uchiumi T
  Scientific reports 9, 14761 (2019).

  • DOI: 10.1038/s41598-019-51266-x

2018

Paper

• "The ribosomal stalk protein is crucial for the action of the conserved ATPase ABCE1"
  Imai H, Abe T, Miyoshi T, Nishikawa S, Ito K, Uchiumi T
  Nucleic acids research 46, 7820-7830 (2018).

  • DOI: 10.1093/nar/gky619

2017

Paper

• "Binding of translation elongation factors to individual copies of the archaeal ribosomal stalk protein aP1 assembled onto aP0"
  Honda T, Imai H, Suzuki, T, Miyoshi T, Ito, K, Uchiumi T
  Biochemical and biophysical research communications 483, 153-158 (2017).

  • DOI: 10.1016/j.bbrc.2016.12.175

2015

Paper

• "Functional role of the C‐terminal tail of the archaeal ribosomal stalk in recruitment of two elongation factors to the sarcin/ricin loop of 23S rRNA"
  Imai H, Miyoshi T, Murakami R, Ito K, Ishino Y, Uchiumi T
  Genes to Cells 20, 613-624 (2015).

  • DOI: 10.1111/gtc.12256