2024

Paper

• "Enhanced Broad-Spectrum Efficacy of an L2-Based mRNA Vaccine Targeting HPV Types 6, 11, 16, 18, with Cross-Protection Against Multiple Additional High-Risk Types"
  Kosuke Tsukamoto, Akio Yamashita, Masatoshi Maeki, Manabu Tokeshi, Hirotatsu Imai, Akira Fukao, Toshinobu Fujiwara, Koji Okudera, Nobuhisa Mizuki, Kenji Okuda, Masaru Shimada
  Vaccines 12, 1239 (2024).

  • DOI: 10.3390/vaccines12111239
• "Metabolic RNA Labeling and Translating Ribosome Affinity Purification for Measurement of Nascent RNA Translation"
  Hirotatsu Imai, Akio Yamashita
  Bio-protocol 14, 20, e5091 (2024).

  • DOI: 10.21769/BioProtoc.5091

2023

Paper

• "Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification"
  Hirotatsu Imai, Daisuke Utsumi, Hidetsugu Torihara, Kenzo Takahashi, Hidehito Kuroyanagi, Akio Yamashita
  Nucleic Acids Research 51, 11, e76 (2023).

  • URL
  • DOI: 10.1093/nar/gkad545

2022

Paper

• "The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation"
  Lei Yang, Ka-Ming Lee, Conny Wing-Heng Yu, Hirotatsu Imai, Andrew Kwok-Ho Choi, David K Banfield, Kosuke Ito, Toshio Uchiumi, Kam-Bo Wong
  Nucleic Acids Research 50, 5335–5348 (2022).

  • DOI: 10.1093/nar/gkac292

2020

Paper

• "Arabidopsis ROOT PHOTOTROPISM2 Is a Light-Dependent Dynamic Modulator of Phototropin1"
  Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito, Tatsuya Sakai
  The Plant Cell 32, 2004-2019 (2020).

  • DOI: 10.1105/tpc.19.00926
• "Direct visualization of translational GTPase factor pool formed around the archaeal ribosomal P-stalk by high-speed AFM"
  Hirotatsu Imai, Toshio Uchiumi, Noriyuki Kodera
  Proceedings of the National Academy of Sciences 117, 32386-32394 (2020).

  • DOI: 10.1073/pnas.2018975117

2019

Paper

• "Switch of the interactions between the ribosomal stalk and EF1A in the GTP-and GDP-bound conformations"
  Kei Maruyama, Hirotatsu Imai, Momoko Kawamura, Sonoko Ishino, Yoshizumi Ishino, Kosuke Ito, Toshio Uchiumi
  Scientific reports 9, 14761 (2019).

  • DOI: 10.1038/s41598-019-51266-x

2018

Paper

• "The ribosomal stalk protein is crucial for the action of the conserved ATPase ABCE1"
  Hirotatsu Imai, Takaya Abe, Tomohiro Miyoshi, Shu-ichi Nishikawa, Kosuke Ito, Toshio Uchiumi
  Nucleic acids research 46, 7820-7830 (2018).

  • DOI: 10.1093/nar/gky619

2017

Paper

• "Binding of translation elongation factors to individual copies of the archaeal ribosomal stalk protein aP1 assembled onto aP0"
  Takayoshi Honda, Hirotatsu Imai, Takahiro Suzuki, Tomohiro Miyoshi, Kosuke Ito, Toshio Uchiumi
  Biochemical and biophysical research communications 483, 153-158 (2017).

  • DOI: 10.1016/j.bbrc.2016.12.175

2015

Paper

• "Functional role of the C‐terminal tail of the archaeal ribosomal stalk in recruitment of two elongation factors to the sarcin/ricin loop of 23S rRNA"
  Hirotatsu Imai, Tomohiro Miyoshi, Ryo Murakami, Kosuke Ito, Yoshizumi Ishino, Toshio Uchiumi
  Genes to Cells 20, 613-624 (2015).

  • DOI: 10.1111/gtc.12256